Mutant farnesyltransferase beta subunit of Saccharomyces cerevisiae that can substitute for geranylgeranyltransferase type I beta subunit.
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چکیده
منابع مشابه
Mutant farnesyltransferase subunit of Saccharomyces cerevisiae that can substitute for geranylgeranyltransferase type
The protein farnesyltransferase (PFT) 13-subunit gene of Saccharomyces cerevisiae, DPRI, was randomly mutagenized by PCR to construct a mutant DPRI gene library on a high-copy plasmid. The library was screened for suppression of the temperature sensitivity conferred by a mutation in the protein geranylgeranyltransferase type I (PGGT-I) 18-subunit gene, CALl. A mutant DPRI gene was identified wh...
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Protein prenyltransferases catalyze the covalent attachment of isoprenoid lipids (farnesyl or geranylgeranyl) to a cysteine near the C terminus of their substrates. This study explored the specificity determinants for interactions between the farnesyltransferase of Saccharomyces cerevisiae and its protein substrates. A series of substitutions at amino acid 149 of the farnesyltransferase beta-su...
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More than 100 proteins necessary for eukaryotic cell growth, differentiation, and morphology require posttranslational modification by the covalent attachment of an isoprenoid lipid (prenylation). Prenylated proteins include members of the Ras, Rab, and Rho families, lamins, CENPE and CENPF, and the g subunit of many small heterotrimeric G proteins. This modification is catalyzed by the protein...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1995
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.92.5.1704